Machine learning and metabolic modelling assisted implementation of a novel process analytical technology in cell and gene therapy manufacturing
Cell culture A serum-free, suspension-adapted, HEK293T-based LVV PCL, used to produce recombinant, VSV-G pseudotyped, HIV-1-GFP based LVVs, was generated previously from an adherent HEK293T cell line, provided by Oxford Biomedica; all LVV encoding genetic elements were stably integrated into the host-cell genome. The PCL was cultured in FreeStyle 293 Expression Medium (Gibco, Thermo Fisher Scientific, …